![]() ![]() ![]() A positive result is revealed after 24-48 hours incubation at 55☌, by a heavy growth of organisms causing turbidity in the broth. Tryptone Soya Broth is recommended in the USP (Casein soya bean digest broth) for the recovery of organisms after sterilisation processes 1. Oxoid laboratory tests have shown that Tryptone Soya Broth has a greater ability to resuscitate heated spores of Geobacillus stearothermophilus than Dextrose Tryptone Broth. Tryptone Soya Broth is especially suitable for the tube dilution method of antibiotic susceptibility testing. Five to 10ml of blood may be added to 50ml of medium. Anticoagulants such as `liquid’† (sodium polyanethyl sulphonate) or sodium citrate may be added to the broth prior to sterilisation. The superior growth-promoting properties of Tryptone Soya Broth make it especially useful for the isolation of organisms from blood or other body fluids. For this purpose, the broth (with added agar) should be used soon after sterilisation, or, heated and cooled just before inoculation. ![]() The addition of a small amount of agar renders the broth suitable for the cultivation of obligatory anaerobes, such as Clostridium spp. Cultures should be examined at frequent intervals, as maximum growth is reached earlier than with less nutritious media and the phase of decline consequently begins sooner. Tryptone Soya Broth may be used for the cultivation of aerobes and facultative anaerobes, including some fungi. Tryptone Soya Broth conforms to formulations detailed in various international pharmacopoeias 1,4,5,6. Due to the inclusion of both Tryptone and Soya Peptone, the medium will support a luxuriant growth of many fastidious organisms without the addition of serum, etc. Sterilise by autoclaving at 121☌ for 15 minutes.Ī highly nutritious versatile medium which is recommended for general laboratory use. TRYPTONE SOYA BROTH (Casein soya bean digest medium) EP/USP/JP/BPĪ highly nutritious general purpose medium for the growth of bacteria and fungiĪdd 30g to 1 litre of water (purified as required), mix well and distribute into final containers. ![]()
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